تاثیر استرس بی حرکتی مزمن و ژل رویال بر میزان هورمون های استروئیدی، کورتیزول و تغییرات بافتی رحم در موش سوری ماده

Background and purpose: Chronic stress is known as a state that disrupts the body homeostasis that could cause significant behavioral and hormonal changes. In this study the changes in estrogen, progesterone, cortisol, and testosterone hormonal level and histological changes in uterus tissue induced by immobilization stress were studied. Also, the protective effect of royal jelly was investigated. Materials and methods: In this study, 40 female mice were divided into 5 groups of 8 including: 1- control, 2- mice subjected to 63 days of 2 hour immobilization stress daily, Groups 3, 4, and 5 that were subjected to immobilization stress and received royal jelly orally at the dose of 50, 100 and 200 mg/kg body weight, respectively. At the end of treatment, the mice were sacrificed, serum was separated and the level of serum cortisol, estrogen, progesterone and testosterone was measured using ELISA method. The uterine horn was also removed and after preparing tissue sections and performing H&E staining they were studied by light microscopy. Results: The levels of serum estrogen, cortisol and testosterone hormones of stressed group increased compared to those of the control group, while the amount of progesterone decreased. In royal jelly- treated groups, the levels of estrogen, testosterone and cortisol reduced but the level of progesterone increased. Also, the study of uterine horn tissue indicated histological changes in stressed group compared to those of the controls. In groups treated with royal jelly, these changes were lower and the uterine tissue was similar to that of the control group. Conclusion: Royal Jelly improved the hormonal and histological changes due to immobilization stress